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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
29/09/2014 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
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ISSN : |
0717-3458 |
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DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
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Thesagro : |
CITRUS; CYDIA. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
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024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCITRUS
650 $aCYDIA
700 1 $aMARCONDES, J.A.
700 1 $aPICCHI, S.C.
700 1 $aSTUCHI, E.S.
700 1 $aFADEL, A.L.
700 1 $aLAIA, M.L.
700 1 $aLEMOS, M.V.F.
773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
04/09/2018 |
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Tipo de producción científica : |
Documentos |
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Autor : |
SILVEIRA, C.; MONTOSSI, F.; CUADRO, W.; LUZARDO, S.; CIAPPESONI, C.; DE BARBIERI, I.; RODRÍGUEZ, A.; BOTTERO, D.; LIMA, D.; CÁCERES, I.; BRITO, G.; SAN JULIAN, R.; COSTALES, J.; DE SOUZA, G. |
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Afiliación : |
CAROLINA INES SILVEIRA ROJAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WASHINGTON ROBIN CUADRO LOPEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS IGNACIO DE BARBIERI ETCHEBERRY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DAVID WILLIAMS LIMA GONZALEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO WALTER BRITO DIAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO SAN JULIAN SANCHEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO CESAR COSTALES NUÑEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO DE SOUZA CAMARGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Efecto de diferentes sistemas de alimentación sobre la performance animal, calidad de la canal y carne y valor nutritivo de la carne de corderos Corriedale y sus cruzas Merino Dohne pastoreando una pastura de Plantago lanceolata cv. Tonic y Lotus Corniculatus cv. INIA Draco. |
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Fecha de publicación : |
2008 |
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Fuente / Imprenta : |
ln: Día de campo, INIA Tacuarembó, Unidad Experimental Glencoe, Setiembre, 2008. Alternativas de intensificación, especialización, diversificación y valorización de la ganadería ovina y bovina en basalto. Tacuarembó (Uruguay): INIA, 2008. |
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Páginas : |
p. 13-16 |
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Idioma : |
Español |
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Contenido : |
Objetivo general: Evaluar el efecto de diferentes sistemas de alimentación sobre la performance animal, calidad de la canal y la carne, con particular énfasis en las características nutricionales de la carne y la salud humana, en corderos Corriedale puros y Corriedale x Merino Dohne. |
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Thesagro : |
CARNE; CORDERO; LOTUS CORNICULATUS; PLANTAGO LANCEOLATA; RAZAS (ANIMALES); SISTEMAS DE ALIMENTACION. |
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Asunto categoría : |
L02 Alimentación animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/4374/1/GLENCOE-2008SetGuia-de-actividad-Visita-guiada.pdf
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Marc : |
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100 1 $aSILVEIRA, C.
245 $aEfecto de diferentes sistemas de alimentación sobre la performance animal, calidad de la canal y carne y valor nutritivo de la carne de corderos Corriedale y sus cruzas Merino Dohne pastoreando una pastura de Plantago lanceolata cv. Tonic y Lotus Corniculatus cv. INIA Draco.
260 $aln: Día de campo, INIA Tacuarembó, Unidad Experimental Glencoe, Setiembre, 2008. Alternativas de intensificación, especialización, diversificación y valorización de la ganadería ovina y bovina en basalto. Tacuarembó (Uruguay): INIA$c2008
300 $ap. 13-16
520 $aObjetivo general: Evaluar el efecto de diferentes sistemas de alimentación sobre la performance animal, calidad de la canal y la carne, con particular énfasis en las características nutricionales de la carne y la salud humana, en corderos Corriedale puros y Corriedale x Merino Dohne.
650 $aCARNE
650 $aCORDERO
650 $aLOTUS CORNICULATUS
650 $aPLANTAGO LANCEOLATA
650 $aRAZAS (ANIMALES)
650 $aSISTEMAS DE ALIMENTACION
700 1 $aMONTOSSI, F.
700 1 $aCUADRO, W.
700 1 $aLUZARDO, S.
700 1 $aCIAPPESONI, C.
700 1 $aDE BARBIERI, I.
700 1 $aRODRÍGUEZ, A.
700 1 $aBOTTERO, D.
700 1 $aLIMA, D.
700 1 $aCÁCERES, I.
700 1 $aBRITO, G.
700 1 $aSAN JULIAN, R.
700 1 $aCOSTALES, J.
700 1 $aDE SOUZA, G.
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INIA Tacuarembó (TBO) |
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